Overview

  • Product name
    Anti-WIF1 antibody - C-terminal
    See all WIF1 primary antibodies
  • Description
    Rabbit polyclonal to WIF1 - C-terminal
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human WIF1, conjugated to KLH.

  • Positive control
    • 293 cell lysates transiently transfected with the WIF1 gene.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 42 kDa.
IHC-P 1/10 - 1/50.

Target

Images

  • All lanes : Anti-WIF1 antibody - C-terminal (ab71205) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the WIF1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab71205 at 1/50 dilution staining WIF1- C-terminal in human Lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

This product has been referenced in:
  • Huang S  et al. Coexpression of SFRP1 and WIF1 as a prognostic predictor of favorable outcomes in patients with colorectal carcinoma. Biomed Res Int 2014:256723 (2014). IHC . Read more (PubMed: 24949429) »
  • Delmas AL  et al. WIF1 is a frequent target for epigenetic silencing in squamous cell carcinoma of the cervix. Carcinogenesis 32:1625-33 (2011). Read more (PubMed: 21873353) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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