Anti-WIF1 antibody [MM0602-6D27] (ab89935)

Overview

  • Product nameAnti-WIF1 antibody [MM0602-6D27]
    See all WIF1 primary antibodies
  • Description
    Mouse monoclonal [MM0602-6D27] to WIF1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein Human WIF1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • ClonalityMonoclonal
  • Clone numberMM0602-6D27
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/20 - 1/100.

Target

Anti-WIF1 antibody [MM0602-6D27] images

  • ab89935 staining WIF1 in human stomach tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer pH 6.0, prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-WIF1 antibody [MM0602-6D27] (ab89935)

ab89935 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Jun 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"