Overview

  • Product nameAnti-WIF1 antibody - N-terminal
    See all WIF1 primary antibodies
  • Description
    Rabbit polyclonal to WIF1 - N-terminal
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human WIF1, conjugated to KLH.

  • Positive control
    • 293 cell lysates transiently transfected with the WIF1 gene.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 42 kDa.
IHC-P 1/10 - 1/50.

Target

Anti-WIF1 antibody - N-terminal images

  • All lanes : Anti-WIF1 antibody - N-terminal (ab71204) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the WIF1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ab71204 at 1/50 dilution staining WIF1- N-terminal in human Lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-WIF1 antibody - N-terminal (ab71204)

This product has been referenced in:
  • Lee HS  et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . Read more (PubMed: 24376796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Human Tissue sections (follicle)
Specification follicle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Apr 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"