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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody was purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 42 kDa.
1/10 - 1/50.
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Sequence similarities Contains 5 EGF-like domains. Contains 1 WIF domain.
Cellular localization Secreted.
Information by UniProt
Wnt inhibitory factor 1 antibody
Anti-WIF1 antibody - N-terminal images
Western blot - WIF1 antibody - N-terminal (ab71204)
All lanes : Anti-WIF1 antibody - N-terminal (ab71204) at 1/50 dilution Lane 1 : Nontransfected 293 cell lysates Lane 2 : 293 cell lysates transiently transfected with the WIF1 gene Lysates/proteins at 2 µg per lane. Predicted band size : 42 kDa Observed band size : 42 kDa Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-WIF1 antibody - N-terminal(ab71204)
ab71204 at 1/50 dilution staining WIF1- N-terminal in human Lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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