Anti-Wilms Tumor Protein antibody [6F-H2] (ab93050)


  • Product name
    Anti-Wilms Tumor Protein antibody [6F-H2]
    See all Wilms Tumor Protein primary antibodies
  • Description
    Mouse monoclonal [6F-H2] to Wilms Tumor Protein
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Truncated Human Wilms Tumor Protein, corresponding to N terminal amino acids 1 - 181.

  • Positive control
    • Wilm's tumor



Our Abpromise guarantee covers the use of ab93050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/15 - 1/25 in an ABC method. An incubation period of 30 - 60 minutes at room temperature is recommended. Perform heat mediated antigen retrieval, using 10 mM citrate buffer, pH 6.0, before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
    • Tissue specificity
      Expressed in the kidney and a subset of hematopoietic cells.
    • Involvement in disease
      Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
      Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
      Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
      Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
      Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
      Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
    • Sequence similarities
      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 4 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
    • Information by UniProt
    • Database links
    • Alternative names
      • WIT 2 antibody
      • WT 1 antibody
      • AWT1 antibody
      • FWT1 antibody
      • GUD antibody
      • NPHS4 antibody
      • WAGR antibody
      • Wilms tumor 1 antibody
      • Wilms Tumor antibody
      • Wilms tumor protein antibody
      • Wilms' tumor gene antibody
      • Wilms' tumor protein antibody
      • WIT2 antibody
      • WT antibody
      • WT1 antibody
      • WT1_HUMAN antibody
      • WT33 antibody
      see all


    ab93050 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Immunocytochemistry/ Immunofluorescence
    Human Cell (Pluripotent stem cell derived epicardial like cell)
    Yes - 0.5% Triton-X
    Pluripotent stem cell derived epicardial like cell
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jul 13 2016

    I hope that you are well.

    About a month ago you have kindly contacted us for the bulk purchase of ab93050. I had confirmed by email about the availability of large volume. I am sorry since then I did not heard anything, could you please update...

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    Thank you for contacting us.

    I just spoken to my colleague in laboratory and I can confirm that we have another 4 ml of this lot in stock. Please feel free to use 1 vial first and if you are satisfied buy the rest. In case you would like to bu...

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