Overview

  • Product name
  • Description
    Rabbit polyclonal to WIPF2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 392-419 of Human WIPF2 (NP_573571.1).

  • Positive control
    • 293 cell line lysate; Human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab108132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance
    WIPF2 is a WASP interacting protein (WIP)-related protein. It has been shown that WIPF2 has a role in the WASP-mediated organization of the actin cytoskeleton and is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery.
  • Cellular localization
    Cytoplasm; cytoskeleton. Note: Localized to stress fibers and bundles of actin filaments.
  • Database links
  • Alternative names
    • PP10631 antibody
    • WAS/WASL interacting protein family member 2 antibody
    • WASP binding protein antibody
    • WASP interacting protein related protein antibody
    • WICH antibody
    • WIP and CR16 homologous protein antibody
    • WIP related protein antibody
    • WIPF 2 antibody
    • WIPF2_HUMAN antibody
    • WIRE antibody
    see all

Images

  • ICC/IF image of ab108132 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108132 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-WIPF2 antibody (ab108132) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 46 kDa
  • ab108132, at 1/50 dilution, staining WIPF2 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

ab108132 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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