Overview

  • Product name
    Anti-WIPI2 antibody [2A2]
    See all WIPI2 primary antibodies
  • Description
    Mouse monoclonal [2A2] to WIPI2
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-P, IHC-Fr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    CSALRLDEDSEHPPMILRTD

    , corresponding to C terminal amino acids 418-436 of Human WIPI2 (Isoform WIPI2b).

  • Epitope
    EHPPM
  • Positive control
    • HEK293
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    2A2
  • Isotype
    IgG1
  • Light chain type
    kappa

Applications

Our Abpromise guarantee covers the use of ab105459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with Acetone.
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P.
  • Tissue specificity
    Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
  • Sequence similarities
    Belongs to the WD repeat SVP1 family.
    Contains 3 WD repeats.
  • Cellular localization
    Preautophagosomal structure membrane. Enriched at preautophagosomal structure membranes in response to ptdIns3P.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG18B antibody
    • Atg21 antibody
    • CGI 50 antibody
    • DKFZp434J154 antibody
    • DKFZp686P02188 antibody
    • FLJ12979 antibody
    • FLJ14217 antibody
    • FLJ42984 antibody
    • WD repeat domain phosphoinositide-interacting protein 2 antibody
    • WD repeat domain, phosphoinositide interacting 2 antibody
    • WD40 repeat protein interacting with phosphoinositides 2 antibody
    • WIPI 2 antibody
    • WIPI-2 antibody
    • WIPI2 antibody
    • WIPI2_HUMAN antibody
    • WIPI49 like protein 2 antibody
    • WIPI49-like protein 2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab105459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105459, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Lee MY  et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep 18:48-60 (2017). Human . Read more (PubMed: 27827795) »
  • Jung J  et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. Elife 6:N/A (2017). WB, ICC/IF ; Human . Read more (PubMed: 28195531) »

See all 11 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab105459.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up