Overview

  • Product nameAnti-WIPI2 antibody [2A2]
    See all WIPI2 primary antibodies
  • Description
    Mouse monoclonal [2A2] to WIPI2
  • Tested applicationsSuitable for: ICC/IF, IP, IHC-P, IHC-Fr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    CSALRLDEDSEHPPMILRTD

    , corresponding to C terminal amino acids 418-436 of Human WIPI2 (Isoform WIPI2b).

  • EpitopeEHPPM
  • Positive control
    • HEK293
  • General notesGrowth Conditions: DMEM + 15% FCS

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone number2A2
  • IsotypeIgG1
  • Light chain typekappa

Applications

Our Abpromise guarantee covers the use of ab105459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with Acetone.
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProbable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P.
  • Tissue specificityUbiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
  • Sequence similaritiesBelongs to the WD repeat SVP1 family.
    Contains 3 WD repeats.
  • Cellular localizationPreautophagosomal structure membrane. Enriched at preautophagosomal structure membranes in response to ptdIns3P.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG18B antibody
    • Atg21 antibody
    • CGI 50 antibody
    • DKFZp434J154 antibody
    • DKFZp686P02188 antibody
    • FLJ12979 antibody
    • FLJ14217 antibody
    • FLJ42984 antibody
    • WD repeat domain phosphoinositide-interacting protein 2 antibody
    • WD repeat domain, phosphoinositide interacting 2 antibody
    • WD40 repeat protein interacting with phosphoinositides 2 antibody
    • WIPI 2 antibody
    • WIPI-2 antibody
    • WIPI2 antibody
    • WIPI2_HUMAN antibody
    • WIPI49 like protein 2 antibody
    • WIPI49-like protein 2 antibody
    see all

Anti-WIPI2 antibody [2A2] images

  • Overlay histogram showing HeLa cells stained with ab105459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105459, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-WIPI2 antibody [2A2] (ab105459)

This product has been referenced in:
  • Dooley HC  et al. WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1. Mol Cell 55:238-52 (2014). Read more (PubMed: 24954904) »
  • Manzoni C  et al. Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation. Biochem Biophys Res Commun 441:862-6 (2013). Read more (PubMed: 24211199) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"