Overview

  • Product name
  • Description
    Rabbit polyclonal to WISP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within internal amino acids 135-230 of Human WISP1 (UniProt ID: O95388).

  • Positive control
    • A431 and MCF7 cell lysates; A431 cells; Human HT29 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • CCN 4 antibody
    • CCN family member 4 antibody
    • CCN4 antibody
    • WISP 1 antibody
    • WISP 1c antibody
    • WISP 1i antibody
    • WISP 1tc antibody
    • WISP1c antibody
    • WISP1i antibody
    • WISP1tc antibody
    • WNT 1 induced secreted protein 1 antibody
    • Wnt 1 induced secreted protein antibody
    • WNT 1 inducible signaling pathway protein 1 antibody
    • Wnt 1 signaling pathway protein 1 antibody
    • Wnt-1 inducible signaling pathway protein 1 antibody
    • WNT1 induced secreted protein 1 antibody
    • Wnt1 induced secreted protein antibody
    • WNT1 inducible signaling pathway protein 1 antibody
    • Wnt1 signaling pathway protein 1 antibody
    • WNT1-inducible-signaling pathway protein 1 antibody
    see all

Images

  • Anti-WISP1 antibody (ab155654) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human HT29 xenograft tissue, labeling WISP1 using ab155654 at a 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed A431 cells, labeling WISP1 using ab155654 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155654 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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