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Rabbit polyclonal to WISP1
Synthetic peptide, corresponding to a region within internal amino acids 135-230 of Human WISP1 (UniProt ID: O95388).
A431 and MCF7 cell lysates; A431 cells; Human HT29 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.
CCN 4 antibody
CCN family member 4 antibody
Western blot - Anti-WISP1 antibody (ab155654)
Anti-WISP1 antibody (ab155654) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody (ab155654)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human HT29 xenograft tissue, labeling WISP1 using ab155654 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-WISP1 antibody (ab155654)
Immunofluorescence analysis of methanol-fixed A431 cells, labeling WISP1 using ab155654 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"