Withaferin A from Withania somnifera (ab120644)

Overview

  • Product nameWithaferin A from Withania somnifera
  • Description
    Naturally occurring withanolide/steroidal lactone with anticancer properties
  • Biological descriptionNaturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
  • Purity> 97%

Properties

  • Chemical name(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
  • Molecular weight470.60
  • Chemical structureChemical Structure
  • Molecular formulaC28H38O6
  • CAS Number5119-48-2
  • Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in ethanol
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Withania somnifera

  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab120644 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Withaferin A from Withania somnifera images

    • ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    References for Withaferin A from Withania somnifera (ab120644)

    This product has been referenced in:
    • Zhang X  et al. Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A. Gynecol Oncol 124:606-12 (2012). Read more (PubMed: 22188785) »
    • Mirjalili MH  et al. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 14:2373-93 (2009). Read more (PubMed: 19633611) »

    See 0 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"