Overview

  • Product name
    Anti-WldS antibody
  • Description
    Rabbit polyclonal to WldS
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    CDNIAVRGLHVGQHHQLLP

    (Mouse) conjugated to KLH

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
IHC-Fr
IP
  • Application notes
    ICC/IF: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IHC-P: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IHC-Fr: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IP: 1/20. See Laser, Conforti et al., 2006.
    WB: 1/2000 - 1/5000. A single band is seen at these dilutions. Predicted molecular weight: 43 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The slow Wallerian Degeneration (WldS) gene encodes a novel Ube4b-Nmnat1 chimeric protein (WldS protein). WldS protein confers a neuroprotective effect on axonal and synaptic compartments of neurons from a wide variety of degeneration-inducing stimuli. The chimeric WldS gene occurred as the result of a spontaneous mutation in C57BL/6 mice, resulting in a tandem triplication of a region already present on the distal region of chromosome 4. The triplicated region contains sequences coding for Nmnat1, Rbp7 and Ube4b. The boundaries within the triplicated region result in 2 copies of a fusion gene comprising the N70 terminal amino acids of Ube4b and the entire coding region of Nmnat1 (C Terminal 285 amino acids), linked by 18 amino acids from the 5' untranslated region of Nmnat1 which are not normally expressed.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • slow wallerian degeneration protein antibody
      • Wallerian degeneration protein antibody
      • Wld antibody

    Images

    • All lanes : Anti-WldS antibody (ab94345) at 1/2000 dilution

      Lane 1 : Wild-type mouse brain homogenate
      Lane 2 : WldS mouse brain homogenate


      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References

    ab94345 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab94345.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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