Overview

  • Product nameAnti-WldS antibody
  • Description
    Rabbit polyclonal to WldS
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    CDNIAVRGLHVGQHHQLLP

    (Mouse) conjugated to KLH

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
IHC-Fr
IP
  • Application notesICC/IF: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IHC-P: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IHC-Fr: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
    IP: 1/20. See Laser, Conforti et al., 2006.
    WB: 1/2000 - 1/5000. A single band is seen at these dilutions. Predicted molecular weight: 43 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe slow Wallerian Degeneration (WldS) gene encodes a novel Ube4b-Nmnat1 chimeric protein (WldS protein). WldS protein confers a neuroprotective effect on axonal and synaptic compartments of neurons from a wide variety of degeneration-inducing stimuli. The chimeric WldS gene occurred as the result of a spontaneous mutation in C57BL/6 mice, resulting in a tandem triplication of a region already present on the distal region of chromosome 4. The triplicated region contains sequences coding for Nmnat1, Rbp7 and Ube4b. The boundaries within the triplicated region result in 2 copies of a fusion gene comprising the N70 terminal amino acids of Ube4b and the entire coding region of Nmnat1 (C Terminal 285 amino acids), linked by 18 amino acids from the 5' untranslated region of Nmnat1 which are not normally expressed.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • slow wallerian degeneration protein antibody
      • Wallerian degeneration protein antibody
      • Wld antibody

    Anti-WldS antibody images

    • All lanes : Anti-WldS antibody (ab94345) at 1/2000 dilution

      Lane 1 : Wild-type mouse brain homogenate
      Lane 2 : WldS mouse brain homogenate


      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References for Anti-WldS antibody (ab94345)

    ab94345 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"