• Product name
  • Description
    Rabbit polyclonal to WNK1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from huma WNK1 around the phosphorylation site of threonine 58 (R-H-T-M-D).

  • Positive control
    • EGF-treated 293 cell extract; human breast carcinoma tissue.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab53151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Predicted molecular weight: 251 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20398666


  • Function
    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity
    Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • Involvement in disease
    Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PPP1R167 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • Protein phosphatase 1, regulatory subunit 167 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 1 antibody
    • Serine/threonine protein kinase WNK1 2 antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK lysine deficient protein kinase 1 isoform antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with ab53151 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-WNK1 antibody (ab53151) at 1/300 dilution

    Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with no peptide
    Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with peptide

    Predicted band size : 251 kDa


This product has been referenced in:
  • Park HW  et al. Dynamic regulation of CFTR bicarbonate permeability by [Cl-]i and its role in pancreatic bicarbonate secretion. Gastroenterology 139:620-31 (2010). IHC-Fr ; Human . Read more (PubMed: 20398666) »

See 1 Publication for this product

Customer reviews and Q&As

I'm really sorry but we unfortunately don't. Sorry I cannot help you more.

Thank you for your enquiry. I would predict that a cytoplasmic lysate of human breast tumor tissue will have some WNK1 protein as in tissue sections of human breast carcinoma this protein was detected with the antibody. However, as the lysate is a ho...

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