Recombinant
RabMAb

Anti-WNK1 antibody [EPR2744(3)] (ab181029)

Overview

  • Product name
    Anti-WNK1 antibody [EPR2744(3)]
    See all WNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2744(3)] to WNK1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human WNK1 aa 1850-1950 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H4A3

  • Positive control
    • Human fetal brain, HeLa, 293T and Jurkat cell lysate. 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2744(3)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 250 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Function
    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity
    Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • Involvement in disease
    Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PPP1R167 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • Protein phosphatase 1, regulatory subunit 167 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 1 antibody
    • Serine/threonine protein kinase WNK1 2 antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK lysine deficient protein kinase 1 isoform antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all

Images

  • All lanes : Anti-WNK1 antibody [EPR2744(3)] (ab181029) at 1000

    Lane 1 : Human fetal brain lysate
    Lane 2 : Hela cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 250 kDa
  • Immunofluorescent staining of 293T cells labeling WNK1 with ab181029 at 1/50 dilution (green). DAPI nuclear staining (blue).

References

ab181029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Malignant Schwann Cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
100 µg
Treatment
WNK1 shRNA
Specification
Malignant Schwann Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Submitted Mar 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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