Overview

  • Product name
    Anti-WNK1 (phospho T58) antibody
    See all WNK1 primary antibodies
  • Description
    Rabbit polyclonal to WNK1 (phospho T58)
  • Specificity
    ab53137 detects endogenous levels of WNK1 only when phosphorylated at threonine 58.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).

  • Positive control
    • 293 cell extracts treated with EGF (200ng/ml, 30mins) and human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity
    Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • Involvement in disease
    Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PPP1R167 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • Protein phosphatase 1, regulatory subunit 167 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 1 antibody
    • Serine/threonine protein kinase WNK1 2 antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK lysine deficient protein kinase 1 isoform antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all

Images

  • All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution

    Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
    Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide


    Predicted band size : 251 kDa
  • ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
  • Cheng CJ & Huang CL Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1. J Am Soc Nephrol 22:460-71 (2011). WB ; Human . Read more (PubMed: 21355052) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hek 293T)
Loading amount
60 µg
Specification
Hek 293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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