Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
1/500. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa). Lysates from transfected HEK293 cells expressing Myc-WNK2 as well as endogenous WNK2
Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab30698).
WNK2 a putative tumour suppressor is a member of a family of serine / threonine protein kinases implicated in the hereditary form of hypertension, pseudohypoaldosteronism type II.
mitogen-activated protein kinase kinase kinase antibody
NY CO 43 antibody
Protein kinase lysine deficient 2 antibody
Protein kinase with no lysine 2 antibody
Serine/Threonine protein kinase WNK2 antibody
Serologically defined colon cancer antigen 43 antibody
WNK lysine deficient protein kinase 2 antibody
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Western blot - Anti-WNK2 antibody (ab28852)This image is courtesy of an abreview submitted by Peter Jordan
All lanes : Top blot: anti Myc-Tag antibody; Bottom Blot: ab28852 (1/500)
Lane 1 : Hek293 cell lysate Transfected with empty vector Lane 2 : Hek293 cell lysate Transfected with MYC-WNK1 Lane 3 : Hek293 cell lysate Transfected with MYC-WNK2 Lane 4 : Hek293 cell lysate Transfected with MYC-WNK3 Lane 5 : Hek293 cell lysate Transfected with MYC-WKN4
Secondary All lanes : Goat anti-rabbit IgG conjugated to HRP at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 300 kDa
Anti-tubulin antibody used as loading control for both blots.