Overview

  • Product nameAnti-WNK2 antibody
    See all WNK2 primary antibodies
  • Description
    Mouse monoclonal to WNK2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KGTFTDDLHK LVDEWTSKTV GAAQLKPTLN QLKQTQKLQD MEAQAGWAAP GEARAMTAPR AGVGMPRLPP APGPLSTTVI PGAAPTLSVP TPDPESEKPD , corresponding to amino acids 2118-2218 of Human WNK2

Properties

Applications

Our Abpromise guarantee covers the use of ab57691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceWNK2 a putative tumour suppressor is a member of a family of serine / threonine protein kinases implicated in the hereditary form of hypertension, pseudohypoaldosteronism type II.
    • Cellular localizationCell Membrane and Cytoplasmic
    • Database links
    • Alternative names
      • Antigen NY-CO-43 antibody
      • KIAA1760 antibody
      • mitogen-activated protein kinase kinase kinase antibody
      • NY CO 43 antibody
      • P/OKcl.13 antibody
      • PRKWNK2 antibody
      • Protein kinase lysine deficient 2 antibody
      • Protein kinase with no lysine 2 antibody
      • SDCCAG43 antibody
      • Serine/Threonine protein kinase WNK2 antibody
      • Serologically defined colon cancer antigen 43 antibody
      • WNK lysine deficient protein kinase 2 antibody
      see all

    Anti-WNK2 antibody images

    • WNK2 antibody (ab57691) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
    • Western blot against tagged recombinant protein immunogen using ab57691 WNK2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    • Developed using the ECL technique

      Exposure time : 30 seconds

      Image courtesy of Dr Peter Jordan by Abreview.

    References for Anti-WNK2 antibody (ab57691)

    ab57691 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human embryonic kidney (HEK293) cells)
    Loading amount 400000 cells
    Specification human embryonic kidney (HEK293) cells
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Peter Jordan

    Verified customer

    Submitted Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"