Overview

  • Product name
    Anti-WNK4 antibody
  • Description
    Rabbit polyclonal to WNK4
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 1005-1138 of Human WNK4.

  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity
    Expressed in kidney, colon and skin.
  • Involvement in disease
    Pseudohypoaldosteronism 2B
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by WNK1 and WNK3.
    Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation and increased expression of KCNJ1 at the cell surface. Ubiquitinated by the BCR(KLHL2) complex.
  • Cellular localization
    Cell junction, tight junction. Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • PHA 2B antibody
    • PHA2B antibody
    • PRKWNK 4 antibody
    • PRKWNK4 antibody
    • Protein kinase lysine deficient 4 antibody
    • Protein kinase lysine-deficient 4 antibody
    • Protein kinase with no lysine 4 antibody
    • Serine/threonine protein kinase WNK4 antibody
    • Serine/threonine-protein kinase WNK4 antibody
    • WNK lysine deficient protein kinase 4 antibody
    • Wnk4 antibody
    • WNK4_HUMAN antibody
    see all

Images

  • ab126477, at 1/20 dilution, staining WNK4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Wolley MJ  et al. In Primary Aldosteronism, Mineralocorticoids Influence Exosomal Sodium-Chloride Cotransporter Abundance. J Am Soc Nephrol 28:56-63 (2017). Human . Read more (PubMed: 27381844) »

See 1 Publication for this product

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