Anti-Wnt1 antibody (ab15251)
Key features and details
- Rabbit polyclonal to Wnt1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt1 antibody
See all Wnt1 primary antibodies -
Description
Rabbit polyclonal to Wnt1 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Wnt1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P04628 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (2) |
1/100.
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WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/100. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7471 Human
- Entrez Gene: 22408 Mouse
- Entrez Gene: 24881 Rat
- Omim: 164820 Human
- SwissProt: P04628 Human
- SwissProt: P04426 Mouse
- Unigene: 248164 Human
- Unigene: 1123 Mouse
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Alternative names
- BMND16 antibody
- INT1 antibody
- OI15 antibody
see all
Images
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Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size: 41 kDa
Observed band size: 41 kDa -
ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
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ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (142)
ab15251 has been referenced in 142 publications.
- Yu Y et al. High Fat Diet Induces Kidney Injury via Stimulating Wnt/β-Catenin Signaling. Front Med (Lausanne) 9:851618 (2022). PubMed: 35462998
- Zhang Y et al. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/β-catenin signaling in obstructed kidneys in vivo. J Biol Chem 298:102010 (2022). PubMed: 35525270
- Huang X et al. Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis. BMC Endocr Disord 22:189 (2022). PubMed: 35869471
- Mounir MMF et al. Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study. J Oral Biol Craniofac Res 12:721-726 (2022). PubMed: 36097610
- Xu J et al. Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling. Am J Cancer Res 12:5552-5563 (2022). PubMed: 36628278