The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1.
sELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa.
The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.