Anti-Wnt10a antibody (ab106522)
Key features and details
- Rabbit polyclonal to Wnt10a
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt10a antibody
See all Wnt10a primary antibodies -
Description
Rabbit polyclonal to Wnt10a -
Host species
Rabbit -
Specificity
Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).
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Positive control
- WB: RAW264.7 cell lysate. IHC-P: Human and mouse skeletal muscle tissue; Rat bladder tissue. ICC/IF: Human skeletal muscle cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
Not validated in human. |
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IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. Not validated in human. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. -
Involvement in disease
Defects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Note=Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway.
Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD) [MIM:257980]. OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin.
Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS) [MIM:224750]. SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 80326 Human
- Entrez Gene: 22409 Mouse
- Omim: 606268 Human
- SwissProt: Q9GZT5 Human
- SwissProt: P70701 Mouse
- Unigene: 121540 Human
- Unigene: 5130 Mouse
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Alternative names
- FLJ14301 antibody
- OODD antibody
- Protein Wnt-10a antibody
see all
Images
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Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa -
Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 µg/ml in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 µg/ml in immunohistochemical analysis.
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Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.
Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. -
Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab106522 has been referenced in 13 publications.
- Sun X et al. Diffuse Large B-Cell Lymphoma Promotes Endothelial-to-Mesenchymal Transition via WNT10A/Beta-Catenin/Snail Signaling. Front Oncol 12:871788 (2022). PubMed: 35494062
- Kasacka I et al. Wnt/β-catenin signaling in the adrenal glands of rats in various types of experimental hypertension. Endocr Connect 11:N/A (2022). PubMed: 35904223
- Zhao B et al. Wnt10a/β-catenin signalling is involved in kindlin-1-mediated astrocyte activation in a chronic construction injury rat model. Eur J Neurosci 54:7409-7421 (2021). PubMed: 34618385
- Zhu L et al. WNT/ß-catenin pathway activation via Wnt1 overexpression and Axin1 downregulation correlates with cadherin-catenin complex disruption and increased lymph node involvement in micropapillary-predominant lung adenocarcinoma. J Thorac Dis 12:5906-5915 (2020). PubMed: 33209423
- Piotrowska Z et al. Comparative Assessment of the WNT/ß-Catenin Pathway, CacyBP/SIP, and the Immunoproteasome Subunit LMP7 in Various Histological Types of Renal Cell Carcinoma. Front Oncol 10:566637 (2020). PubMed: 33330038