Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt10b
  • Specificity
    ab70816 only recognizes the longer isoform of Wnt10b and will not cross-react with Wnt10a.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    15 amino acid peptide from near the center of human Wnt10b (NP_003385).

  • Positive control
    • Human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity
    Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • Involvement in disease
    Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
  • Sequence similarities
    Belongs to the Wnt family.
  • Developmental stage
    Infant brain has higher levels of WNT10B than adult brain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-10b antibody
    • Protein Wnt-12 antibody
    • SHFM6 antibody
    • wingless type MMTV integration site family, member 10B antibody
    • WN10B_HUMAN antibody
    • WNT 10B protein antibody
    • WNT 12 antibody
    • Wnt10b antibody
    • wnt12 antibody
    see all

Images

  • Lane 1 : Anti-Wnt10b antibody (ab70816) at 2 µg/ml
    Lane 2 : Anti-Wnt10b antibody (ab70816) at 4 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 93 kDa. We are unsure as to the identity of these extra bands.
  • ab70816 at 2.5 µ/ml staining Wnt10 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • ICC/IF image of ab70816 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Richards MH  et al. Dynamic interaction between astrocytes and infiltrating PBMCs in context of neuroAIDS. Glia 63:441-51 (2015). Read more (PubMed: 25331637) »
  • Richards MH  et al. Porcupine is not required for the production of the majority of Wnts from primary human astrocytes and CD8+ T cells. PLoS One 9:e92159 (2014). WB . Read more (PubMed: 24647048) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3-L1 preadipocytes)
Loading amount
16.6 µg
Specification
3T3-L1 preadipocytes
Treatment
With vehicle (DMSO) or Tunicamycin (10 µg/ml for 6h or 24h)
Gel Running Conditions
Reduced Denaturing (Invitrogen NuPAGE 4-14% Bis-Tris Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. William Cawthorn

Verified customer

Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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