Overview

  • Product nameAnti-Wnt11 antibody
    See all Wnt11 primary antibodies
  • Description
    Rabbit polyclonal to Wnt11
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates for WB, HeLa cells for IF and Lung SCC for IHC-P.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/100 - 1/250.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificityExpressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.
  • Sequence similaritiesBelongs to the Wnt family.
  • Post-translational
    modifications
    Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • HWNT11 antibody
    • Protein Wnt-11 antibody
    • Wingless-related MMTV integration site 11 antibody
    • Wingless-type MMTV integration site family, member 1 antibody
    • Wingless-type MMTV integration site family, member 11 antibody
    • Wingless-type MMTV integration site family, member 11B antibody
    • wnt-11 antibody
    • wnt11 antibody
    • WNT11_HUMAN antibody
    • wnt11b antibody
    see all

Anti-Wnt11 antibody images

  • Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 39 kDa
  • Immunohistochemical analysis of paraffin-embedded lung SCC, using ab96730 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96730 at 1/200 dilution. Lower image merged with DNA probe.

References for Anti-Wnt11 antibody (ab96730)

This product has been referenced in:
  • Matise LA  et al. Lack of transforming growth factor-ß signaling promotes collective cancer cell invasion through tumor-stromal crosstalk. Breast Cancer Res 14:R98 (2012). Read more (PubMed: 22748014) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult ovary and uterus)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification Adult ovary and uterus
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Sep 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"