Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt16
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: KRKMRRREKD QRKIPIHKDD LLYVNKSPNY CVEDKKLGIP GTQGRECNRT, corresponding to C terminal amino acids 264-313 of Human Wnt16

  • Positive control
    • HepG2 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1:312500.

Target

Images

  • Anti-Wnt16 antibody (ab64461) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Richards MH  et al. Porcupine is not required for the production of the majority of Wnts from primary human astrocytes and CD8+ T cells. PLoS One 9:e92159 (2014). WB . Read more (PubMed: 24647048) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab64461 Anti-Wnt16 antibody has never been tested in functional blocking experiments. All tested and guaranteed applications are specified on our datasheets, and the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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