Overview

  • Product nameAnti-Wnt16 antibody
    See all Wnt16 primary antibodies
  • Description
    Rabbit polyclonal to Wnt16
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: KRKMRRREKD QRKIPIHKDD LLYVNKSPNY CVEDKKLGIP GTQGRECNRT, corresponding to C terminal amino acids 264-313 of Human Wnt16

  • Positive control
    • HepG2 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1:312500.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificityIsoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt 16 precursor antibody
    • Protein Wnt-16 antibody
    • Wingless type MMTV integration site family member 16 antibody
    • WNT16 antibody
    • WNT16_HUMAN antibody
    see all

Anti-Wnt16 antibody images

  • Anti-Wnt16 antibody (ab64461) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-Wnt16 antibody (ab64461)

ab64461 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab64461 Anti-Wnt16 antibody has never been tested in functional blocking experiments. All tested and guaranteed applications are specified on our datasheets, and the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"