Recombinant
RabMAb

Anti-Wnt16 antibody [EPR6064] (ab109437)

Overview

  • Product name
    Anti-Wnt16 antibody [EPR6064]
    See all Wnt16 primary antibodies
  • Description
    Rabbit monoclonal [EPR6064] to Wnt16
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Wnt16

  • Positive control
    • LnCaP, HepG2, Raji, and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 6.05 x 10 -11 M Learn more about KD
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6064
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Tissue specificity
      Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
    • Sequence similarities
      Belongs to the Wnt family.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein Wnt 16 precursor antibody
      • Protein Wnt-16 antibody
      • Wingless type MMTV integration site family member 16 antibody
      • WNT16 antibody
      • WNT16_HUMAN antibody
      see all

    Anti-Wnt16 antibody [EPR6064] images

    • All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Wnt16 antibody [EPR6064] (ab109437)

    This product has been referenced in:
    • Andres-Mateos E  et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other . Read more (PubMed: 23155423) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
    Specification
    Wholemount 3-5 days post fertilisation
    Username

    Dr. Chrissy Hammond

    Verified customer

    Submitted Jul 23 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver, Spleen)
    Specification
    Liver, Spleen
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Thank you for this data.

    After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came ...

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    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased withi...

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    Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence...

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    DISCOUNT CODE: 00000

    Expiration date:04. January 2013

    Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to you...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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