The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
Tissue specificityIsoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.
Western blot - Anti-Wnt2b antibody (ab50575)
All lanes : Anti-Wnt2b antibody (ab50575) at 1.25 µg/ml
Lane 1 : Jusrkat cell lysate Lane 2 : Jusrkat cell lysate with blocking peptide at 4 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size : 41 kDa
Western blot - Anti-Wnt2b antibody (ab50575)This image is courtesy of an anonymous Abreview
All lanes : Anti-Wnt2b antibody (ab50575) at 1/1000 dilution (in TBST + 5% Milk Powder for 3 hours at 21°C)
Lane 1 : Whole tissue lysate of Mouse (FVB) liver Lane 2 : Whole tissue lysate of Mouse (FVB) liver Lane 3 : Whole tissue lysate of Mouse (FVB) liver Lane 4 : Whole tissue lysate of Mouse (BL6) liver Lane 5 : Lysate of HepG2 clls
Lysates/proteins at 20 µg per lane.
Secondary An HRP-conjugated Goat anti-rabiit IgG polyclonal at 1/10000 dilution
Klaus C et al. Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity. World J Gastroenterol20:14855-64 (2014).
WB, IP, IHC
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