Overview

  • Product nameAnti-Wnt2b antibody
    See all Wnt2b primary antibodies
  • Description
    Rabbit polyclonal to Wnt2b
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: AVQVMATQDG ANFTAARQGY RRATRTDLVY FDNSPDYCVL DKAAGSLGTA, corresponding to internal sequence amino acids 253-302 of Human Wnt2b.

  • Positive control
    • Jurkat cell lysate and human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab50575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
  • Tissue specificityIsoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-13 antibody
    • Protein Wnt-2b antibody
    • Wingless type MMTV integration site family, member 13 antibody
    • Wingless type MMTV integration site family, member 2B antibody
    • WNT13 antibody
    • Wnt2b antibody
    • WNT2B_HUMAN antibody
    • XWNT2 antibody
    • XWNT2, Xenopus, homolog of antibody
    see all

Anti-Wnt2b antibody images

  • Anti-Wnt2b antibody (ab50575) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.
  • All lanes : Anti-Wnt2b antibody (ab50575) at 1.25 µg/ml

    Lane 1 : Jusrkat cell lysate
    Lane 2 : Jusrkat cell lysate with blocking peptide at 4 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 41 kDa
  • All lanes : Anti-Wnt2b antibody (ab50575) at 1/1000 dilution (in TBST + 5% Milk Powder for 3 hours at 21°C)

    Lane 1 : Whole tissue lysate of Mouse (FVB) liver
    Lane 2 : Whole tissue lysate of Mouse (FVB) liver
    Lane 3 : Whole tissue lysate of Mouse (FVB) liver
    Lane 4 : Whole tissue lysate of Mouse (BL6) liver
    Lane 5 : Lysate of HepG2 clls

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabiit IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step : 5% Milk for 16 hours at 4°C

    See Abreview

References for Anti-Wnt2b antibody (ab50575)

This product has been referenced in:
  • Ma M  et al. Wnt signaling in form deprivation myopia of the mice retina. PLoS One 9:e91086 (2014). WB ; Mouse . Read more (PubMed: 24755605) »
  • Klaus C  et al. Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity. World J Gastroenterol 20:14855-64 (2014). WB, IP, IHC ; Human . Read more (PubMed: 25356045) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Sep 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"