Recombinant
RabMAb

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)

Overview

  • Product name
    Anti-Wnt3 and Wnt3a antibody [EPR4920(2)]
  • Description
    Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Wnt3 and Wnt3a aa 150-250 (Cysteine residue). The exact sequence is proprietary. SwissProt: P56703 and P56704
    Database link: P56704

  • Positive control
    • CHO cells transfected with Human Wnt3a.
  • General notes

    This antibody was developed as part of a collaboration with Karl Willert’s lab from the University of California at San Diego.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      Proto-oncogene Wnt-3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Protein Wnt-3a: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
    • Database links

    Images

    • All lanes : Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612) at 1/10000 dilution

      Lane 1 : CHO cell lysate, non-transfected
      Lane 2 : CHO cell lysate, transfected with Human Wnt3a

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    References

    This product has been referenced in:
    • Lippert A  et al. Single-Molecule Imaging of Wnt3A Protein Diffusion on Living Cell Membranes. Biophys J 113:2762-2767 (2017). Read more (PubMed: 29262368) »
    • Shukla S  et al. Cucurbitacin B inhibits the stemness and metastatic abilities of NSCLC via downregulation of canonical Wnt/ß-catenin signaling axis. Sci Rep 6:21860 (2016). WB . Read more (PubMed: 26905250) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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