Overview

  • Product nameAnti-Wnt3 antibody
    See all Wnt3 primary antibodies
  • Description
    Goat polyclonal to Wnt3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    CGRGHNTRTEKRKEK

    , corresponding to C terminal amino acids 315-329 of Human Wnt3.

  • Positive control
    • WB: Human brain lysate IHC-P: Human adrenal, breast and thyroid tissues

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab116222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/2000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Involvement in diseaseDefects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Int 4 antibody
    • INT4 antibody
    • MGC131950 antibody
    • MGC138321 antibody
    • MGC138323 antibody
    • Proto-oncogene Int-4 homolog antibody
    • Proto-oncogene Wnt-3 antibody
    • wingless type MMTV integration site family member 3 antibody
    • Wnt 3 proto oncogene protein antibody
    • WNT 3 proto oncogene protein precursor antibody
    • wnt3 antibody
    • WNT3_HUMAN antibody
    see all

Anti-Wnt3 antibody images

  • Anti-Wnt3 antibody (ab116222) at 1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 40 kDa


    Exposure time : 1 hour
  • ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Wnt3 antibody (ab116222)

ab116222 has not yet been referenced specifically in any publications.

Product Wall


The blocking peptide which can be used with ab116222 is Wnt3 peptide (315-329) (ab50436).

Thank you for contacting us. Here are the links to our Whole mount staining protocols - Protocols and troubleshooting tips for whole mount immunohistochemical staining: http://www.abcam.com/index.html?pageconfig=resource&rid=11330 and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"