Anti-Wnt3 antibody (ab50341)
Key features and details
- Goat polyclonal to Wnt3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3 antibody
See all Wnt3 primary antibodies -
Description
Goat polyclonal to Wnt3 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken, Dog -
Immunogen
Synthetic peptide:
CGRGHNTRTEKRKEK
, corresponding to internal sequence amino acids 315-329 of Human Wnt3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 2 - 4 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 4 µg/ml. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Involvement in disease
Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 374142 Chicken
- Entrez Gene: 7473 Human
- Entrez Gene: 24882 Rat
- Omim: 165330 Human
- SwissProt: P56703 Human
- Unigene: 445884 Human
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Alternative names
- Int 4 antibody
- INT4 antibody
- MGC131950 antibody
see all
Images
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ab50341 (2µg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
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Anti-Wnt3 antibody (ab50341) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 40 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab50341 has been referenced in 3 publications.
- Luo W et al. c-Myc inhibits myoblast differentiation and promotes myoblast proliferation and muscle fibre hypertrophy by regulating the expression of its target genes, miRNAs and lincRNAs. Cell Death Differ 26:426-442 (2019). PubMed: 29786076
- Tripathy A et al. The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways. PLoS One 13:e0208194 (2018). PubMed: 30513115
- Lee YS et al. Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development. Cell Host Microbe 24:833-846.e6 (2018). PubMed: 30543778