- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-Wnt3a antibody [3A6]
See all Wnt3a primary antibodies - DescriptionMouse monoclonal [3A6] to Wnt3a
- Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).
- Positive control
- A549 cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityAmmonium Sulphate Precipitation
- ClonalityMonoclonal
- Clone number3A6
- IsotypeIgG2a
- Light chain typekappa
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab81614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
- FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
- Tissue specificityModerately expressed in placenta and at low levels in adult lung, spleen, and prostate.
- Sequence similaritiesBelongs to the Wnt family.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 89780 Human
- Omim: 606359 Human
- SwissProt: P56704 Human
- Unigene: 336930 Human
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Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Anti-Wnt3a antibody [3A6] images
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![Western blot - Wnt3a antibody [3A6] (ab81614)](http://a.abcam.com/ps/datasheet/images/81/ab81614/Wnt3a-Primary-antibodies-ab81614-2.jpg)
Western blot - Wnt3a antibody [3A6] (ab81614)Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution + A549 lysate at 20 µg
Secondary
Goat anti-mouse HRP
Developed using the ECL technique
Predicted band size : 39 kDa
Observed band size : 39 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)](http://a.abcam.com/ps/datasheet/images/81/ab81614/Wnt3a-Primary-antibodies-ab81614-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)](http://a.abcam.com/ps/datasheet/images/81/ab81614/Wnt3a-Primary-antibodies-ab81614-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.
References for Anti-Wnt3a antibody [3A6] (ab81614)
This product has been referenced in:
- Meng X et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . Read more (PubMed: 28469782) »
- Chen F et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. J Surg Res 192:298-304 (2014). Read more (PubMed: 25033705) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"