Overview

  • Product name
    Anti-Wnt3a antibody [3A6]
    See all Wnt3a primary antibodies
  • Description
    Mouse monoclonal [3A6] to Wnt3a
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).

  • Positive control
    • A549 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity
    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Images

  • Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution + A549 lysate at 20 µg

    Secondary
    Goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

References

This product has been referenced in:
  • Meng X  et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . Read more (PubMed: 28469782) »
  • Pan LH  et al. Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma. World J Gastroenterol 22:3829-36 (2016). Read more (PubMed: 27076768) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 17 2014

Here are the answers to your questions.


1. Are there high resolution images available for the 2 IHC stainings shown on the website?
-I’ve attached higher resolution pictures as requested.

2. Have any additional sec...

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