Overview

  • Product nameAnti-Wnt3a antibody [3A6]
    See all Wnt3a primary antibodies
  • Description
    Mouse monoclonal [3A6] to Wnt3a
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).

  • Positive control
    • A549 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificityModerately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Anti-Wnt3a antibody [3A6] images

  • Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution + A549 lysate at 20 µg

    Secondary
    Goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

References for Anti-Wnt3a antibody [3A6] (ab81614)

This product has been referenced in:
  • Chen F  et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. J Surg Res 192:298-304 (2014). Read more (PubMed: 25033705) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
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Submitted Jun 17 2014

Here are the answers to your questions.


1. Are there high resolution images available for the 2 IHC stainings shown on the website?
-I’ve attached higher resolution pictures as requested.

2. Have any additional sec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"