Overview

  • Product name
    Anti-Wnt3a antibody [3A6]
    See all Wnt3a primary antibodies
  • Description
    Mouse monoclonal [3A6] to Wnt3a
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).

  • Positive control
    • A549 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity
    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
    • Alternative names
      • Protein Wnt 3a Precursor antibody
      • Protein Wnt-3a antibody
      • Wingless type MMTV integration site family member 3A antibody
      • Wnt 3a antibody
      • wnt3a antibody
      • WNT3A protein antibody
      • WNT3A_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Wnt3a antibody [3A6] (ab81614)
      Western blot - Anti-Wnt3a antibody [3A6] (ab81614)
      All lanes : Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Wnt3a transfected 293T cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat anti-mouse secondary antibody conjugated to HRP

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)

      ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt3a antibody [3A6] (ab81614)

      ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

    References

    This product has been referenced in:
    • Meng X  et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . Read more (PubMed: 28469782) »
    • Pan LH  et al. Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma. World J Gastroenterol 22:3829-36 (2016). Read more (PubMed: 27076768) »

    See all 4 Publications for this product

    Publishing research using ab81614? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Wnt3a antibody [3A6]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jun 17 2014

    I have a few questions regarding this antibody:
    1. Are there high resolution images available for the 2 IHC stainings shown on the website?
    2. Have any additional sections been stained with this antibody and co-stained for cytokeratins?
    ...

    Read More

    Here are the answers to your questions.


    1. Are there high resolution images available for the 2 IHC stainings shown on the website?
    -I’ve attached higher resolution pictures as requested.

    2. Have any additional sec...

    Read More

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