Overview

  • Product nameAnti-Wnt3a antibody
    See all Wnt3a primary antibodies
  • Description
    Rabbit polyclonal to Wnt3a
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Xenopus laevis
    Predicted to work with: Chicken, Human, Zebrafish, a wide range of other species
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Wnt3a.

    (Peptide available as ab23326)

  • Positive control
    • In WB, this antibody gave a positive signal when tested against a partial recombinant protein. IHC-P: Mouse placenta FFPE tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab19925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Can be blocked with Wnt3a peptide (ab23326).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Wnt3a antibody images

  • IHC image of Wnt3a staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19925, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • Anti-Wnt3a antibody (ab19925) at 1 µg/ml + Recombinant mouse Wnt3a, AAs 19-352 with a 16 AA n-terminal non-Wnt3a sequence at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/15000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    ab19925 detects a band of the expected size for recombinant mouse Wnt3a.
  • ICC/IF image of ab19925 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19925, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Wnt3a antibody (ab19925)

This product has been referenced in:
  • Li C & Siragy HM High glucose induces podocyte injury via enhanced (pro)renin receptor-Wnt-ß-catenin-snail signaling pathway. PLoS One 9:e89233 (2014). WB ; Mouse . Read more (PubMed: 24533170) »
  • Kumar S  et al. Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity. BMC Biol 12:44 (2014). WB ; Mouse . Read more (PubMed: 24885675) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (airway tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization No
Specification airway tissue
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2016

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Zebrafish Tissue sections (Whole mount (whole zebrafish larvae) and sections)
Specification Whole mount (whole zebrafish larvae) and sections
Username

Dr. Chrissy Hammond

Verified customer

Submitted Nov 07 2011

Thank you for your enquiry. This antibody is predicted to react in human tissues due to sequence homology, but has never been tested. In addition, the antibody has only been tested in Western blot. It is possible that the antibody will perform wel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"