The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, pH 7.2
Concentration information loading...
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.25 - 1.0 µg/ml. Use 100µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa. The detection limit is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Belongs to the Wnt family.
Secreted > extracellular space > extracellular matrix.