Overview

  • Product name
    Anti-Wnt3a antibody (Biotin)
    See all Wnt3a primary antibodies
  • Description
    Rabbit polyclonal to Wnt3a (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human),>98% pure

  • General notes
    The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes
    A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1.0 µg/ml. Use 100µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa. The detection limit is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
    • Tissue specificity
      Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
    • Sequence similarities
      Belongs to the Wnt family.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein Wnt 3a Precursor antibody
      • Protein Wnt-3a antibody
      • Wingless type MMTV integration site family member 3A antibody
      • Wnt 3a antibody
      • wnt3a antibody
      • WNT3A protein antibody
      • WNT3A_HUMAN antibody
      see all

    References

    This product has been referenced in:
    • Ling L  et al. Synergism between Wnt3a and heparin enhances osteogenesis via a phosphoinositide 3-kinase/Akt/RUNX2 pathway. J Biol Chem 285:26233-44 (2010). Read more (PubMed: 20547765) »
    • Kwon HS  et al. Myocilin is a modulator of Wnt signaling. Mol Cell Biol 29:2139-54 (2009). ELISA ; Mouse . Read more (PubMed: 19188438) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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