Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt4
  • Host species
    Rabbit
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except WNT5A (71%).
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Wnt4 (internal sequence). 14 amino acid peptide.
    Database link: P56705

  • Positive control
    • Human thyroid tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab189037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
  • Involvement in disease
    Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
    Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
    Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC123964 antibody
    • Protein Wnt-4 antibody
    • RP23-246F18.1 antibody
    • SERKAL antibody
    • Wingless type MMTV integration site family member 4 precursor antibody
    • WNT 4 antibody
    • WNT 4 protein precursor antibody
    • WNT4 antibody
    • WNT4_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyoid tissue labeling Wnt4 with ab189037 at 10 µg/ml.

References

ab189037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M citrate buffer (pH 9.0)
Permeabilization
No
Specification
embryonic ovary
Blocking step
BlockAce(Dainippon Pharmaceutical, Osaka, Japan) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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