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Synthetic peptide corresponding to Human Wnt4 aa 211-239 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 54361
Our Abpromise guarantee covers the use of ab91226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Predicted molecular weight: 39 kDa.|
Immunohistochemical analysis of paraformaldehyde-fixed frozen murine thymus tissue sections, labelling Wnt4 with ab91226 at a dilution of 1/20 incubated for 72 hours at 25°C in a 1% BSA & 3% donkey serum solution in PBST. Permeabilsation was with PBST including 0.1% Triton X-100. Blocking was with the BSA & donkey serum ab91226 was diluted in, incubated for 1 hour at 25°C. The secondary used was a donkey anti-rabbit polyclonal Alexa Fluor® 488
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Wnt4 with ab91226. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.
Flow cytometry analysis of MDA-MB468 cells labelling Wnt4 (green) with ab91226 compared to a negative control (blue). A FITC-conjugated goat anit-rabbit IgG was used as the secondary antibody.