Overview

  • Product nameAnti-Wnt4 antibody
    See all Wnt4 primary antibodies
  • Description
    Rabbit polyclonal to Wnt4
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Horse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Wnt4 aa 211-239 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 54361

  • Positive control
    • WB: Mouse bladder tissue lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt: MB468 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab91226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 39 kDa.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
  • Involvement in diseaseDefects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
    Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
    Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC123964 antibody
    • Protein Wnt-4 antibody
    • RP23-246F18.1 antibody
    • SERKAL antibody
    • Wingless type MMTV integration site family member 4 precursor antibody
    • WNT 4 antibody
    • WNT 4 protein precursor antibody
    • WNT4 antibody
    • WNT4_HUMAN antibody
    see all

Anti-Wnt4 antibody images

  • Immunohistochemical analysis of paraformaldehyde-fixed frozen murine thymus tissue sections, labelling Wnt4 with ab91226 at a dilution of 1/20 incubated for 72 hours at 25°C in a 1% BSA & 3% donkey serum solution in PBST. Permeabilsation was with PBST including 0.1% Triton X-100. Blocking was with the BSA & donkey serum ab91226 was diluted in, incubated for 1 hour at 25°C. The secondary used was a donkey anti-rabbit polyclonal Alexa Fluor® 488

    See Abreview

  • Anti-Wnt4 antibody (ab91226) at 1/1000 dilution + Mouse bladder tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 39 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Wnt4 with ab91226. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.

  • ICC/IF image of ab91226 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow cytometry analysis of MDA-MB468 cells labelling Wnt4 (green) with ab91226 compared to a negative control (blue). A FITC-conjugated goat anit-rabbit IgG was used as the secondary antibody.

References for Anti-Wnt4 antibody (ab91226)

This product has been referenced in:
  • Drelon C  et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development. Nat Commun 7:12751 (2016). IHC-P ; Mouse . Read more (PubMed: 27624192) »
  • Wang L  et al. Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae. PLoS One 8:e56025 (2013). ICC/IF ; Horse . Read more (PubMed: 23405249) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Whole embryo- trigeminal ganglion)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM Citrate Buffer pH6.0
Permeabilization No
Specification Whole embryo- trigeminal ganglion
Blocking step heat-inactivated donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative modified Carnoy's
Username

Dr. Clare Baker

Verified customer

Submitted Nov 23 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (adult thymus)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification adult thymus
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted May 13 2013

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