Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (GVSPQGFQWS GCSDNIAYGV AFSQSFVDVR ERSKGASSSR ALMNLHNNEA) of Human Wnt4 (NP_110388).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
  • Involvement in disease
    Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
    Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
    Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC123964 antibody
    • Protein Wnt-4 antibody
    • RP23-246F18.1 antibody
    • SERKAL antibody
    • Wingless type MMTV integration site family member 4 precursor antibody
    • WNT 4 antibody
    • WNT 4 protein precursor antibody
    • WNT4 antibody
    • WNT4_HUMAN antibody
    see all

Images

  • Anti-Wnt4 antibody (ab94742) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa

References

ab94742 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Whole embryo- olfactory bulb)
Permeabilization
No
Specification
Whole embryo- olfactory bulb
Blocking step
heat-inactivated donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Clare Baker

Verified customer

Submitted Nov 23 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Whole embryo)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM Citrate Buffer pH6.0
Permeabilization
No
Specification
Whole embryo
Blocking step
heat-inactivated donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
modified Carnoy's
Username

Dr. Clare Baker

Verified customer

Submitted Apr 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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