Anti-Wnt5a antibody [6F2] (ab110073)

Overview

  • Product nameAnti-Wnt5a antibody [6F2]
    See all Wnt5a primary antibodies
  • Description
    Mouse monoclonal [6F2] to Wnt5a
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified recombinant fragment of Human Wnt5a, expressed in E. Coli.

  • Positive control
    • Human prostate, thyroid and uterus tissues; PC12 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.
IHC-P 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • Tissue specificityExpression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
  • Sequence similaritiesBelongs to the Wnt family.
  • Post-translational
    modifications
    Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
    Glycosylation is necessary for secretion but not for activity.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • hWNT 5A antibody
    • hWNT5A antibody
    • Protein Wnt 5a antibody
    • Protein Wnt-5a antibody
    • Protein Wnt5a antibody
    • Wingless type MMTV integration site family member 5A antibody
    • Wnt 5a antibody
    • WNT 5A protein antibody
    • WNT 5A protein precursor antibody
    • WNT5A antibody
    • WNT5A protein precursor antibody
    • WNT5A_HUMAN antibody
    see all

Anti-Wnt5a antibody [6F2] images

  • All lanes : Anti-Wnt5a antibody [6F2] (ab110073) at 1/500 dilution

    Lane 2 : HEK 293 cell lysate
    Lane 3 : WNT5A-hIgGFc transfected HEK 293 cell lysate


    Predicted band size : 42 kDa
  • Confocal immunofluorescence analysis of PC12 cells, using a 1/200 dilution of ab110073 in green, and showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • ab110073, at a 1/200 dilution, staining Wnt5a in formalin fxed, paraffin embedded Human prostate by Immunohistochemistry.
  • ab110073, at a 1/200 dilution, staining Wnt5a in formalin fxed, paraffin embedded Human thyroid by Immunohistochemistry.
  • ab110073, at a 1/200 dilution, staining Wnt5a in formalin fxed, paraffin embedded Human uterus by Immunohistochemistry.

References for Anti-Wnt5a antibody [6F2] (ab110073)

ab110073 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer ph 9,0
Permeabilization Yes - Commercial Wash Buffer with Tween
Specification Stomach
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Jun 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"