Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt5a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide within Human Wnt5a aa 185-213 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P41221

  • Positive control
    • Flow Cytometry: HeLa cells. IHC-P: Human lung carcinoma tissue. WB: Human, mouse and rat heart lysate. HeLa and PANC-1 cell lysate. Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • Tissue specificity
    Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
  • Sequence similarities
    Belongs to the Wnt family.
  • Post-translational
    modifications
    Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
    Glycosylation is necessary for secretion but not for activity.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • hWNT 5A antibody
    • hWNT5A antibody
    • Protein Wnt 5a antibody
    • Protein Wnt-5a antibody
    • Protein Wnt5a antibody
    • Wingless type MMTV integration site family member 5A antibody
    • Wnt 5a antibody
    • WNT 5A protein antibody
    • WNT 5A protein precursor antibody
    • WNT5A antibody
    • WNT5A protein precursor antibody
    • WNT5A_HUMAN antibody
    see all

Images

  • All lanes : Anti-Wnt5a antibody (ab174963) at 1/2000 dilution

    Lane 1 : Human heart lysate
    Lane 2 : PANC-1 (Human pancreatic epithelial carcinoma cell line) whole cell lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 42 kDa

  • Immunohistochemical analysis of formalin fixed and paraffin embedded human lung carcinoma labeling Wnt5a with ab174963 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of Hela (Human epithelial cell line from cervix adenocarcinoma) cells (green histogram) compared to a negative control cell (blue histogram) labeling Wnt5a with ab174963 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-Wnt5a antibody (ab174963) at 1/100 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

    Predicted band size: 42 kDa

  • All lanes : Anti-Wnt5a antibody (ab174963) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : PANC-1 (Human pancreatic epithelial carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 42 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

References

ab174963 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain tissue)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Arjang Salehi

Verified customer

Submitted Jul 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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