Synthetic peptide corresponding to Human Wnt5a. Corresponding to a region between amino acids 190 and 230 of Human Wnt5a
Our Abpromise guarantee covers the use of ab72583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21341990|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21857966|
ab72583 staining Wnt5a in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab72583 staining Wnt5a in Mouse rib tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 20% Serum for 1 hour at 19°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 647-conjugated anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.