Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt5a
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Monkey
    Predicted to work with: Xenopus laevis, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human Wnt5a. Corresponding to a region between amino acids 190 and 230 of Human Wnt5a

  • Positive control
    • Human, rat and mouse heart lysates. Human breast tumour tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21341990
IHC-FoFr Use at an assay dependent concentration. PubMed: 21857966

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • Tissue specificity
    Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
  • Sequence similarities
    Belongs to the Wnt family.
  • Post-translational
    modifications
    Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
    Glycosylation is necessary for secretion but not for activity.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • hWNT 5A antibody
    • hWNT5A antibody
    • Protein Wnt 5a antibody
    • Protein Wnt-5a antibody
    • Protein Wnt5a antibody
    • Wingless type MMTV integration site family member 5A antibody
    • Wnt 5a antibody
    • WNT 5A protein antibody
    • WNT 5A protein precursor antibody
    • WNT5A antibody
    • WNT5A protein precursor antibody
    • WNT5A_HUMAN antibody
    see all

Images

  • All lanes : Anti-Wnt5a antibody (ab72583) at 0.5 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate with immunising peptide
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat heart lysate


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • ab72583 staining Wnt5a in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab72583 staining Wnt5a in Mouse rib tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 20% Serum for 1 hour at 19°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 647-conjugated anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.

    See Abreview

  • Immunohistochemical analysis of mouse hippocampus tissue, staining Wnt5a with ab72583.

    Tissue was fixed with paraformaldehyde before being sectioned on a microtome. The sections were permeabilized in Triton X-100 in PBS for 1 hour at room temperature. Samples were incubated with primary antibody (5 µg/ml in blocking solution) at 4°C overnight. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody.

References

This product has been referenced in:
  • Sun X  et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). Read more (PubMed: 28147310) »
  • Zhao G  et al. The Soluble VEGF Receptor sFlt-1 Contributes to Impaired Neovascularization in Aged Mice. Aging Dis 8:287-300 (2017). Read more (PubMed: 28580185) »

See all 24 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviductal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Specification
oviductal tissues
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 23 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (aortic smooth muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
aortic smooth muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (spina cord)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
spina cord
Blocking step
1xRapid block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (ovarian cancer)
Specification
ovarian cancer
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 23 2013

Thank you for contacting us and for providing this information.

The band which you have been seeing around 37kDa is the specific band for Wnt5a as we have shown on the datasheet for this product. We have seen non specific bands similar to what...

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Thanks you very much for your email.

We have soldmany unitsof this antibody and we havn't recieved any complaint from other customers. So I have checked the used protocolwhich seems fine to me so I am sorry I am unable to find the exact reason...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The positive control we recommend is human, mouse or rat heart lysates and lysates of human breast tissues. Also, I am attaching our ques...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Rib)
Specification
Rib
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

Submitted Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary human osteoblasts)
Loading amount
20 µg
Specification
Primary human osteoblasts
Treatment
Lipopolysaccharide (1µg/ml, 48h) with or without Dexamethasone (10 nM, 48 h) or Compound A (10 nM, 48 h)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Martina Rauner

Verified customer

Submitted Apr 29 2010

1-10 of 11 Abreviews or Q&A

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