Recombinant
RabMAb

Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)

Overview

  • Product name
    Anti-Wnt5a antibody [EPR12698] - C-terminal
    See all Wnt5a primary antibodies
  • Description
    Rabbit monoclonal [EPR12698] to Wnt5a - C-terminal
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Wnt5a aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P41221

  • Positive control
    • Human melanoma lysates, Human breast cancer lysate ; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab179824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/20.
WB 1/100 - 1/1000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
    • Tissue specificity
      Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
    • Sequence similarities
      Belongs to the Wnt family.
    • Post-translational
      modifications
      Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
      Glycosylation is necessary for secretion but not for activity.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • hWNT 5A antibody
      • hWNT5A antibody
      • Protein Wnt 5a antibody
      • Protein Wnt-5a antibody
      • Protein Wnt5a antibody
      • Wingless type MMTV integration site family member 5A antibody
      • Wnt 5a antibody
      • WNT 5A protein antibody
      • WNT 5A protein precursor antibody
      • WNT5A antibody
      • WNT5A protein precursor antibody
      • WNT5A_HUMAN antibody
      see all

    Images

    • Immunofluorescent staining of HeLa cells labeling Wnt5a using ab179824 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Wnt5a with purified ab179824 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824) at 1/1000 dilution

      Lane 1 : Human breast cancer lysate
      Lane 2 : Human melanoma lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 42 kDa

      Blocking/Diluting buffer 5% NFDM/TBST

      Exposure time: Lane 1: 3 minutes 
      Lane 2: 1 minute

       

    References

    ab179824 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Human serum in 0.25% casein solution as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Jan 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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