Rabbit monoclonal [EPR12698] to Wnt5a - C-terminal
ICC/IF, Flow Cyt, WBmore details Unsuitable for:
IHC-P or IP
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Wnt5a aa 300 to the C-terminus. The exact sequence is proprietary. Database link: P41221
Human melanoma lysates, Human breast cancer lysate ; HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
Belongs to the Wnt family.
Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding. Glycosylation is necessary for secretion but not for activity.
Secreted > extracellular space > extracellular matrix.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Wnt5a with purified ab179824 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
All lanes : Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824) at 1/1000 dilution
Lane 1 : Human breast cancer lysate Lane 2 : Human melanoma lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution