Overview

  • Product nameAnti-Wnt5b antibody
    See all Wnt5b primary antibodies
  • Description
    Rabbit polyclonal to Wnt5b
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: LRNESTGSLG TQGRLCNKTS EGMDGCELMC CGRGYNQFKS VQVERCHCKF, corresponding to C terminal amino acids 289-338 of Human Wnt5b.

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-Wnt5b antibody images

  • Anti-Wnt5b antibody (ab66616) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Wnt5b antibody (ab66616)

ab66616 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66616.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"