Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt5b
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (CLRNESTGSL GTQGRLCNKT SEGMDGCELM CCGRGYNQFK SVQVERCHCK) of Human Wnt5b (NP_110402).

  • Positive control
    • Human fetal small intestine lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Wnt5b antibody (ab94914) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
  • ab94914 staining Wnt5b in skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab94914 staining Wnt5b in uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab94914 staining Wnt5b in testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Richards MH  et al. Dynamic interaction between astrocytes and infiltrating PBMCs in context of neuroAIDS. Glia 63:441-51 (2015). Read more (PubMed: 25331637) »
  • Kato S  et al. Mesenchymal-transitioned cancer cells instigate the invasion of epithelial cancer cells through secretion of WNT3 and WNT5B. Cancer Sci 105:281-9 (2014). WB . Read more (PubMed: 24344732) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton-X
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Jun 23 2017

Gracias por contactarnos.

Para la detección del colágeno I en borrego mediante WB, el anticuerpo ab23446 está testado y garantizado por nuestra garantía Abpromise:

http://www.abcam.com/Collagen-I-antibody...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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