Recombinant
RabMAb

Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

Overview

  • Product name
    Anti-Wnt5b antibody [EPR7135(B)]
    See all Wnt5b primary antibodies
  • Description
    Rabbit monoclonal [EPR7135(B)] to Wnt5b
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human Wnt5b.

  • Positive control
    • HT-1376 cell lysate, HepG2 cell lysate, 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7135(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Sequence similarities
      Belongs to the Wnt family.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC2648 antibody
      • Protein Wnt-5b antibody
      • Wingless type MMTV integration site family, member 5B antibody
      • WNT 5B antibody
      • WNT 5B protein antibody
      • Wnt family member 5B antibody
      • Wnt5b antibody
      • WNT5B protein antibody
      • WNT5B_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution

      Lane 1 : HT-1376 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 40 kDa

    References

    ab124818 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124818.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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