Overview

  • Product nameAnti-Wnt7a antibody
    See all Wnt7a primary antibodies
  • Description
    Rabbit polyclonal to Wnt7a
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Wnt7a.

  • Positive control
    • In Western Blot, ab100792 gave a positive signal in Human kidney tissue lysate. This antibody gave a positive result in ICC in HepG2 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).

Abcam recommends using 5% BSA as the blocking agent.

ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.
  • Tissue specificityExpression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
  • Involvement in diseaseDefects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
    Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-7a antibody
    • Protein Wnt-7a precursor antibody
    • Proto oncogene Wnt7a protein antibody
    • proto-oncogene wnt7a protein antibody
    • wingless-type MMTV integration site family, member 7A antibody
    • WNT7A antibody
    • WNT7A_HUMAN antibody
    see all

Anti-Wnt7a antibody images

  • Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab100792 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100792, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.

References for Anti-Wnt7a antibody (ab100792)

This product has been referenced in:
  • Long K  et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl ; Mouse . Read more (PubMed: 26838601) »
  • Suo L  et al. Dietary vitamin A regulates wingless-related MMTV integration site signaling to alter the hair cycle. Exp Biol Med (Maywood) 240:618-23 (2015). Read more (PubMed: 25361771) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Gel Running Conditions Reduced Denaturing (10% GEL)
Loading amount 30 µg
Specification C2C12
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 15 2016

Thank you for your reply.

I contacted with my lab colleagues who confirmed this lot has successfully passed our QC controls, and as with previous batches, it should be detecting a band at ˜40KDa, rather than 48KDa.

Please find attac...

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Thank you for taking the time to complete our questionnaire.

The predicted band size for this protein is 39KDa, however, attending at our in-house tests, the observed band size appears at approximately 41KDa, and other unknown additional band...

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Thank you for calling Abcam. I have done the alignment between the sequences you sent and the immunogen sequences for the antibodies we discussed. For DVL1, there is 75% match between the sequence you sent and the immunogen sequence used for ab106914. ...

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