Anti-Wnt7a antibody (ab100792)
Key features and details
- Rabbit polyclonal to Wnt7a
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Wnt7a antibody
See all Wnt7a primary antibodies -
Description
Rabbit polyclonal to Wnt7a -
Host species
Rabbit -
Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant human Wnt7a protein (ab116171) can be used as positive control in WB. In Western Blot, ab100792 gave a positive signal in Human kidney tissue lysate, human and mouse kidney lysate, HEPg2 cells, COLO 205 whole cell lysate and human colon tumor tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).
Abcam recommends using 5% BSA as the blocking agent. |
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa). Abcam recommends using 5% BSA as the blocking agent. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. -
Tissue specificity
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. -
Involvement in disease
Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 749914 Chimpanzee
- Entrez Gene: 101146237 Gorilla
- Entrez Gene: 7476 Human
- Entrez Gene: 22421 Mouse
- Omim: 601570 Human
- SwissProt: Q1KYL1 Chimpanzee
- SwissProt: Q1KYK7 Gorilla
- SwissProt: O00755 Human
see all -
Alternative names
- Protein Wnt-7a antibody
- Protein Wnt-7a precursor antibody
- Proto oncogene Wnt7a protein antibody
see all
Images
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ab100792 staining Wnt7a in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab100792 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Wnt7a antibody (ab100792) at 1 µg/ml
Lane 1 : Human Kidney lysate
Lane 2 : Mouse Kidney lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : COLO 205 whole cell lysate
Lane 5 : Human Colon tumour tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 39 kDaExposure: 4 minutes
Blocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 41 kDa
Additional bands: 50 kDa, 27kDa
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Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab100792 has been referenced in 19 publications.
- Yang L et al. Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cells. Int J Mol Med 47:N/A (2021). PubMed: 33846764
- Zhang R et al. lncRNA BCAR4 sponges miR-370-3p to promote bladder cancer progression via Wnt signaling. Int J Mol Med 45:578-588 (2020). PubMed: 31894304
- Taxier LR et al. Dickkopf-1 blocks 17ß-estradiol-enhanced object memory consolidation in ovariectomized female mice. Horm Behav 114:104545 (2019). PubMed: 31228421
- Wang L et al. miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a. Oncol Lett 17:3219-3226 (2019). PubMed: 30867752
- Vallon M et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). PubMed: 30304675