The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Tissue specificityModerately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
FormWnt7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid modified and are ligands for members of the frizzled family of receptors, which mediate cell-cell communication during development. Wnt7b is thought to modulate several important signaling pathways in the lung which results in the coordinated proliferation of adjacent epithelial and mesenchymal cells to stimulate organ growth.
Protein Wnt-7b antibody
Wingless related MMTV integration site 7B antibody
Wingless type MMTV integration site family member 7B antibody
Anti-Wnt7b antibody images
Western blot - Wnt7b antibody (ab67771)
All lanes : Anti-Wnt7b antibody (ab67771) at 1/500 dilution
Lane 1 : WNT7B transfected 293T cell lysate Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution