Overview

  • Product name
  • Description
    Rabbit polyclonal to Wnt7b
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (TTLPKFREVG HLLKEKYNAA VQVEVVRASR LRQPTFLRIK QLRSYQKPME) of Human Wnt7b (NP_478679).

  • Positive control
    • Human fetal kidney lysate This antibody gave a positive result when used in the following methanol fixed cell lines: sw480

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.Can be blocked with Wnt7b peptide (ab188529). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity
    Moderately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Form
    Wnt7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid modified and are ligands for members of the frizzled family of receptors, which mediate cell-cell communication during development. Wnt7b is thought to modulate several important signaling pathways in the lung which results in the coordinated proliferation of adjacent epithelial and mesenchymal cells to stimulate organ growth.
  • Alternative names
    • Protein Wnt-7b antibody
    • Wingless related MMTV integration site 7B antibody
    • Wingless type MMTV integration site family member 7B antibody
    • WNT antibody
    • WNT7B antibody
    • WNT7B_HUMAN antibody
    see all

Images

  • ICC/IF image of ab94915 stained SW480 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94915 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Wnt7b antibody (ab94915) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling Wnt7b with ab94915 at 4-8µg/ml.

References

This product has been referenced in:
  • Sun X  et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). Read more (PubMed: 28147310) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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