Overview

  • Product nameAnti-Wnt8a antibody
    See all Wnt8a primary antibodies
  • Description
    Rabbit polyclonal to Wnt8a
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Wnt8a.

  • Positive control
    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Heart; E14 Embryonic Brain; E16 Embryonic Brain; E18 Embryonic Brain; E12 Embryonic Brain and Spinal Cord.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-8a antibody
    • Protein Wnt-8d antibody
    • wingless type MMTV integration site family, member 8A antibody
    • WNT 8A antibody
    • Wnt 8d antibody
    • wnt8a antibody
    • WNT8A_HUMAN antibody
    • WNT8D antibody
    see all

Anti-Wnt8a antibody images

  • All lanes : Anti-Wnt8a antibody (ab110672) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 4 : E16 Ms Embryo Brain Tissue Lysate
    Lane 5 : E18 Ms Embryo Brain Tissue Lysate
    Lane 6 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 14 kDa,87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-Wnt8a antibody (ab110672)

ab110672 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E8.5 mouse embryo section)
Specification E8.5 mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v TritonX100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Nov 16 2012

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