The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Tissue specificityExpression is restricted to the brain, and more specifically to the forebrain.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115532 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Brain, Basal Nucleus of Meynert (formalin-fixed, paraffin-embedded) stained with ab115532 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-Wnt8b antibody (ab115532)
has not yet been referenced specifically in any publications.
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