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Rabbit polyclonal to Wnt8b
HumanPredicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
A region within synthetic peptide: KCHGVSGSCT TQTCWLQLPE FREVGAHLKE KYHAALKVDL LQGAGNSAAG, corresponding to internal sequence amino acids 181-230 of Human Wnt8b
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Expression is restricted to the brain, and more specifically to the forebrain.
Belongs to the Wnt family.
Secreted > extracellular space > extracellular matrix.
Information by UniProt
- Protein Wnt-8b antibody
- Wingless related MMTV integration site 8b antibody
- Wingless type MMTV integration site family, member 8B antibody
Western blot - Anti-Wnt8b antibody (ab66307)
Anti-Wnt8b antibody (ab66307) at 1.25 µg/ml (5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
39 kDaObserved band size:
32 kDa (why is the actual band size different from the predicted?
)Additional bands at:
80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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