Overview

  • Product nameAnti-Wnt9b antibody
    See all Wnt9b primary antibodies
  • Description
    Rabbit polyclonal to Wnt9b
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Wnt9b.

    (Peptide available as ab74751.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Liver (Mouse), Kidney (Mouse) And the following whole cell lysate: HepG2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab69287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).

Target

  • RelevanceWnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
  • Cellular localizationSecreted: extracellular space: extracellular matrix.
  • Database links
  • Alternative names
    • MGC124412 antibody
    • Protein Wnt 9b precursor antibody
    • Protein Wnt-14b antibody
    • Protein Wnt-9b antibody
    • Wingless type MMTV integration site 9B antibody
    • Wingless type MMTV integration site family member 15 antibody
    • Wingless type MMTV integration site family member 9B antibody
    • Wnt 14b antibody
    • Wnt-15 antibody
    • Wnt14b antibody
    • Wnt15 antibody
    • Wnt9b wingless type MMTV integration site family member 9B antibody
    see all

Anti-Wnt9b antibody images

  • All lanes : Anti-Wnt9b antibody (ab69287) at 1 µg/ml

    Lane 1 : HepG2 Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Wnt9b antibody (ab69287)

ab69287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"