Overview

  • Product name
    Anti-WSCD2 antibody
  • Description
    Rabbit polyclonal to WSCD2
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 61-170 of Human WSCD2.

  • Positive control
    • Human colon tissue; Human plasma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the WSCD family.
    Contains 2 WSC domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933413A10Rik antibody
    • C530024P05Rik antibody
    • Gm450 antibody
    • WSC domain containing 2 antibody
    • WSC domain containing protein 2 antibody
    • WSC domain-containing protein 2 antibody
    • WSCD 2 antibody
    • WSCD2 antibody
    • WSCD2_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121392 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121392, at 1/25 dilution, staining WSCD2 in paraffin-embedded Human colon tissue by Immunohistochemistry, showing cytoplasmic positivity, with a granular pattern, in glandular cells.
  • All lanes : Anti-WSCD2 antibody (ab121392) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References

ab121392 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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