WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)

Overview

  • Product name
    WST-1 Assay Reagent - Cell Proliferation (ready to use)
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    WST-1 Cell Proliferation Reagent (ready-to-use) (ab155902) provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at λ=440 nm.


    This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.


    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

  • Notes

    Application: 

    • Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc.
    • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
    • Assessment of physiological mediators and antibodies that inhibit cell growth.
  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab155902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:
  • Senathilake KS  et al. Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro. Exp Parasitol 177:13-21 (2017). Human . Read more (PubMed: 28351683) »
  • Cohen JE  et al. An innovative biologic system for photon-powered myocardium in the ischemic heart. Sci Adv 3:e1603078 (2017). Rat . Read more (PubMed: 28630913) »

See all 5 Publications for this product

Customer reviews and Q&As

cell proliferation in mice splenocytes

Below average Excellent 5/5 (Ease of Use)
Abreviews
- cell concentration should be optimized
- reagent volume should be adapted
- Incubation time should be optimized
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Abcam user community

Verified customer

Submitted Oct 26 2017

Sensitive and reliable reagent.

Good Excellent 5/5 (Ease of Use)
Abreviews
The starting colour of the reagent was different to the pink starting colour I have seen with WST-1 reagent from a different company. However, this did not seem to affect the sensitivity or reliability of the assay and I obtained good, reproducible results.
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Abcam user community

Verified customer

Submitted Jan 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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