• Product name
    WST-1 Cell Proliferation Assay Kit
    See all Cell proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
  • Assay time
    4h 00m
  • Product overview

    WST-1 Cell Proliferation Assay Kit (ab65473) provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in a microtiter plate.
    Visit our FAQs page for tips and troubleshooting.


    This kit was previously called Quick Cell Proliferation Assay Kit

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Relevance
    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.


Our Abpromise guarantee covers the use of ab65473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


References for WST-1 Cell Proliferation Assay Kit (ab65473)

This product has been referenced in:
  • Jungnickel C  et al. Cigarette smoke-induced disruption of pulmonary barrier and bacterial translocation drive tumor-associated inflammation and growth. Am J Physiol Lung Cell Mol Physiol 309:L605-13 (2015). Read more (PubMed: 26209273) »
  • Shanks K  et al. Neuroimmune semaphorin 4D is necessary for optimal lung allergic inflammation. Mol Immunol 56:480-7 (2013). Read more (PubMed: 23911404) »

See all 6 Publications for this product

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The absorption measured at the reference wavelength value is subtracted from each reading. This is to normalize the readings against a non changing value. One wavelength reports the change in a...

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The assay kit has not been designed to use with a standard curve as such. You can indeed reference your readings if you need to. Any cell type that is actively dividing in culture can be used as a positive control. Any cell type that is treated with a...

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The primary difference between the quick cell proliferiation assay (ab65473) and quick cell plus proliferation assay (ab65475) is that the ab65475 is more sensitive and more stable.

Thank you for contacting us and your interest in our products.

The Quick Cell Proliferation Assay Kit (ab65473) and Quick Cell Proliferation Assay Kit II (ab65475) should not be affected by serum in your culture medium. Any differences from th...

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Thank you for your inquiry. I have asked the laboratory with your question. Indeed, the laboratory recommend just that if the cells are cultured in different volume of culture medium, increase or decrease the amount of WST-1/ECS solution correspond...

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ab65474 - enables to perform 2500 assays instead of 500 (like ab65473) and includes a premade stop solution (unlike ab65473). Also the kits include different tetrazolium salts (WST or WST-1 reagent). More details cannot be given out due to proprietary...

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